Epimorphin (EPIM), also known as syntaxin 2 (STX2), is a type IV transmembrane protein that is a member of the syntaxin family of t SNARE (target Soluble NSF Attachment REceptor) membrane fusion proteins. Intracellular Epimorphin functions as a vesicle fusion protein, but extracellular forms that are active in morphogenesis are also found. Human Epimorphin cDNA encodes 289 amino acids (aa) including a coiled coil domain (aa 35 101), a potential cell recognition sequence (aa 105 123), a sequence important for membrane transduction (aa 141 150), a t SNARE domain (aa 191 252), and a C terminal transmembrane domain (aa 265 288). Within aa 1 188, human Epimorphin shares 89%, 88%, 94% and 95% aa sequence homology with mouse, rat, bovine and porcine Epimorphin, respectively. Potential isoforms of 287 and 277 aa show C terminal substitutions that do and do not contain hydrophobic sequences, respectively. Epimorphin has no signal sequence, but cell stress or Ca2+ influx induces plasma membrane crossing, with the assistance of annexin A2 and synapatotagmin 1. A soluble, extracellular 30 kDa form of Epimorphin is produced from the membrane–associated 34 kDa form by cleavage between E245 and H246. Intracellular 70 kDa and 150 kDa complexes are presumed to be dimers and tetramers, respectively. Epimorphin produced by mesenchymal cells influences morphogenesis of epithelia in the breast, kidney, intestine, lung, pancreas, liver, skin and intestines. For cells such as lung, mammary or pancreatic epithelia, soluble Epimorphin promotes tubulogenesis or hollow sphere formation in vitro, while epithelia plated on Epimorphin or Epimorphin producing cells exhibit alpha v integrin dependent adhesion and branching morphogenesis. Mice genetically lacking Epimorphin are sterile due to abnormal spermatogenesis and testicular development. Both endogenous and exogenous Epimorphin are shown to protect cells from oxidative stress.
22.4 kDa, under reducing conditions; 22.9 & 45.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Epimorphin/Syntaxin 2 Protein (rp184938) - SDS-PAGE 3 μg/lane of Recombinant Human Epimorphin/Syntaxin 2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 22.4 kDa under reducing conditions and 22.9 & 45.2 kDa under non-reducing conditions.
ZJ25F0116922
危险品化学品经营许可证（带存储）