Immobilized Recombinant Human Thymidine Kinase 1 Protein (rp184947) at 2.0 μg/mL can bind Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) with the EC50 of 149.0 ng/mL.
Thymidine kinases are 2'-deoxythymidine kinases that phosphorylate deoxythymidine and generate deoxythymidine 5’-phosphate. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Two forms of thymidine kinase are present in mammalian cells, TK1 and TK2. TK1 is cell cycle-dependent and is present in the cytoplasm only in anticipation of cell division; whereas TK2 is located in mitochondria and is cell cycle-independent. TK1 is synthesized by the cell during the S phase of cell division and is degraded after cell division is completed. TK1 normally occurs in tissue as a dimer and can be converted to more active tetrameric form by ATP binding. TK1 is feedback inhibited by thymidine triphosphate, the product of the further phosphorylation of thymidine. Because tumor cells replicate much more frequently than normal cells and requires faster DNA synthesis and higher TK1 activity, TK1 is a cancer maker especially for hematologic malignancies. In clinical chemistry TK1 is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease. In addition, thymidine kinase is required for the action of many antiviral drugs. The enzymatic activity of recombinant human TK1 is measured using a phosphatase-coupled method.
Post-translational: Phosphorylated on Ser-13 in mitosis. Phosphorylation of Ser-13 by CDK1 during mitosis reduces homotetramerization and catalytic efficiency when DNA replication is complete and intracellular TK1 is still present at a high level (PubMed:14697231, PubMed:9575153). Polyubiquitinated. Postmitosis, ubiquitination leads to proteasomal degradation. The KEN box sequence located at the C-terminal region targets for degradation by the anaphase promoting complex (APC/C) activated and rate-limited by FZR1.
生物活性
Immobilized Recombinant Human Thymidine Kinase 1 Protein (rp184947) at 2.0 μg/mL can bind Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) with the EC50 of 149.0 ng/mL.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
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Recombinant Human Thymidine Kinase 1 Protein (rp184947) - ELISA Immobilized Recombinant Human Thymidine Kinase 1 Protein (rp184947) at 2.0 μg/mL can bind Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) with the EC50 of 149.0 ng/mL.
Recombinant Human Thymidine Kinase 1/TK1 Protein (rp184947)- SDS-PAGE 3 μg/lane of Recombinant Human Thymidine Kinase 1/TK1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 26.5 & 50.6 & 74.1 kDa under reducing conditions and 24.2 & 42.1 & 72.9 kDa under non-reducing conditions. Monomer and tetramer were formed at C206 and other sites. Both dimer and tetramer combine with GTP.
ZJ25F0218374
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