Em:670nm,Ex:565nm 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
PE-Cy5 is a tandem fluorophore comprising R-Phycoerythin and a red-shifted cyanine dye. The resonance energy transfer dye Cy5-Phycoerythrin (Cy5PE) is an extremely bright dye, primarily because of the very low autofluorescence in the region where it fluoresces. It is easily excited at 488 nm, and emits at 680 nm; however, it is also well excited at 633 nm (HeNe laser), and thus using Cy5PE reagents in two laser-system requires interlaser compensation.
Many applications and instruments were developed specifically for PE-Cy5. It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumentation available, PE-Cy5 is also well suited for immunohistochemistry (IHC) and Western blot applications.
PE-Cy5 (rp185192) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (PE-Cy5) (rp188522) at 0.05μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
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