Endothelial PAS domain-containing protein 1 | EPAS-1 | Basic-helix-loop-helix-PAS protein MOP2 | Class E basic helix-loop-helix protein 73 (bHLHe73) | HIF-1-alpha-like factor (HLF) | Hypoxia-inducible factor 2-alpha (HIF-2-alpha | HIF2-alpha) | Member of
规格或纯度
无载体,His标签,≥90%(SDS-PAGE),See COA
生化机理
Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity).
Post-translational: In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human HIF-2 alpha Protein (rp186522) - SDS-PAGE 3 μg/lane of Recombinant Human HIF-2 alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 36.7 & 77.9 kDa under reducing conditions and 35.6 & 68.4-83.2 kDa under non-reducing conditions. HIF-2α's C-terminal transactivation domain (CTAD, residues 543-870) recruits transcriptional coactivators to activate target gene transcription and may adopt multiple conformations under non-reducing conditions.
ZJ25F0319310
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