级别和纯度: 无动物源?无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。Fc标签?Fc 标签级 —— 带有 Fc 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。≥95%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
P52799
蛋白标签
C-hFc
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human EphB4 Protein (rp184032) at 2.0 μg/mL can bind Recombinant Human Ephrin-B2 Protein (rp186546) with the EC50 of 52.41 ng/mL.
无动物源, 无载体, 生物活性, ActiBioPure™, Fc标签, ≥95%(SDS-PAGE), expressed in HEK293; 见COA
生化机理
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
Post-translational: Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
生物活性
Immobilized Recombinant Human EphB4 Protein (rp184032) at 2.0 μg/mL can bind Recombinant Human Ephrin-B2 Protein (rp186546) with the EC50 of 52.41 ng/mL.
52.4-65.8 kDa, under reducing conditions; 118.5-147.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human Ephrin-B2 Protein (rp186546) - Binding Activity
Immobilized Recombinant Human EphB4 Protein (rp184032) at 2.0 μg/mL can bind Recombinant Human Ephrin-B2 Protein (rp186546) with the EC50 of 52.41 ng/mL.
Recombinant Human Ephrin-B2 Protein (rp186546) - SDS-PAGE
3 μg/lane of Recombinant Human Ephrin-B2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 52.4-65.8 kDa under reducing conditions and 118.5-147.8 kDa under non-reducing conditions.
Ephrin-B2 can form the dimers through covalent interaction via disulfide bonds under non-reducing conditions (Uniprot: P52799).