Recombinant Human Ephrin-B2 Protein

货号: rp186546
有货
级别和纯度: 无动物源 ? 无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 Fc标签 ? Fc 标签级 —— 带有 Fc 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。 ≥95%(SDS-PAGE) expressed in HEK293; 见COA
Accession #
P52799
蛋白标签
C-hFc
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human EphB4 Protein (rp184032) at 2.0 μg/mL can bind Recombinant Human Ephrin-B2 Protein (rp186546) with the EC50 of 52.41 ng/mL.
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规格
库存
价格
数量
10μg
rp186546-10μg
期货 Stock Image
¥239.90
50μg
rp186546-50μg
期货 Stock Image
¥679.90
100μg
rp186546-100μg
期货 Stock Image
¥1,089.90
1mg
rp186546-1mg
期货 Stock Image
¥7,999.90
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为什么选择此级别

ActiBioPure™,Fc标签,无动物源,无载体,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Human Ephrin-B2 Protein
规格或纯度
无动物源, 无载体, 生物活性, ActiBioPure™, Fc标签, ≥95%(SDS-PAGE), expressed in HEK293; 见COA
生化机理
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus. Post-translational: Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
生物活性
Immobilized Recombinant Human EphB4 Protein (rp184032) at 2.0 μg/mL can bind Recombinant Human Ephrin-B2 Protein (rp186546) with the EC50 of 52.41 ng/mL.
表达系统
HEK293
种属
人(Human)
氨基酸
1-229 aa
序列
MAVRRDSVWKYCWGVLMVLCRTAISKSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAIEGRMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGV/EVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK​
蛋白标签
C-hFc
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
49.1 kDa
SDS-PAGE
52.4-65.8 kDa, under reducing conditions; 118.5-147.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human Ephrin-B2 Protein (rp186546) - Binding Activity​ 
Immobilized Recombinant Human EphB4 Protein (rp184032) at 2.0 μg/mL can bind Recombinant Human Ephrin-B2 Protein (rp186546) with the EC50 of 52.41 ng/mL.
Recombinant Human Ephrin-B2 Protein (rp186546) - SDS-PAGE 
3 μg/lane of Recombinant Human Ephrin-B2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 52.4-65.8 kDa under reducing conditions and 118.5-147.8 kDa under non-reducing conditions. Ephrin-B2 can form the dimers through covalent interaction via disulfide bonds under non-reducing conditions (Uniprot: P52799).

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

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📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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