Receptor interacting protein 3 | Receptor interacting serine threonine kinase 3 | Receptor interacting serine/threonine protein kinase 3 | Receptor-interacting protein 3 | Receptor-interacting serine/threonine-protein kinase 3 | RIP 3 | RIP like protein k
规格或纯度
无载体,His标签,≥95%(SDS-PAGE),见COA
生化机理
Necroptosis is a form of programmed necrotic cell death that is rapidly emerging as an important pathophysiological pathway in numerous disease states. Necroptosis is dependent on receptor-interacting protein kinase 3 (RIPK3), a protein shown to play an important role in experimental models of critical illness. RIPK3 promotes kidney tubular injury via mitochondrial dysfunction. The FAO-dependent RIPK3 mediates pathogenesis of acute lung injury.
Post-translational: (Microbial infection) Proteolytically cleaved by S.flexneri OspD3 within the RIP homotypic interaction motif (RHIM), leading to its degradation and inhibition of necroptosis.RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (PubMed:19524513).Autophosphorylated following interaction with ZBP1 (By similarity).
Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3 (PubMed:11734559, PubMed:19524512).Autophosphorylates at Ser-227 following activation by ZBP1: phosphorylation at these sites is a hallmark of necroptosis and is required for binding MLKL (PubMed:22265413).Phosphorylation at Thr-182 is important for its kinase activity, interaction with PELI1 and PELI1-mediated 'Lys-48'-linked polyubiquitination and for its ability to mediate TNF-induced necroptosis (PubMed:29883609).Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B (PubMed:21931591).Polyubiquitinated with 'Lys-48'-linked chains by PELI1 leading to its subsequent proteasome-dependent degradation. Ubiquitinated by STUB1 leading to its subsequent proteasome-dependent degradation (PubMed:29883609).Deubiquitinated by USP22 (PubMed:33369872).
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human RIPK3 Protein (rp186577) - SDS-PAGE 3 μg/lane of Recombinant Human RIPK3 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 20.5 & 45.0 kDa. The observation of RIPK3 Protein as both dimers and monomers under reducing (R) conditions suggests that a portion of the protein forms dimers. This dimerization may be mediated by (or stabilized by) disulfide bonds (Uniprot: Q9Y572).
ZJ25F1029219
危险品化学品经营许可证（带存储）