Cyclin-dependent kinase 2 (CDK2) belongs the Ser/Thr protein kinase family. CDK2 is highly parallel to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is limited to the G1-S phase, and is vital for cell cycle G1/S phase transition. The CDK2 protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 activity is also regulated by protein phosphorylation.
Post-translational: Phosphorylated at Thr-160 by CDK7 in a CAK complex (PubMed:28666995). Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A. Nitrosylated after treatment with nitric oxide (DETA-NO).
生物活性
Immobilized Recombinant Human CDK2 Protein (rp188224) at 1.0 μg/mL can bind CDK2 Mouse mAb (Ab095749) with the EC50 of 62.4 ng/mL.
Store at -20℃ for 6 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
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Recombinant Human CDK2 Protein (rp188224) - ELISA Immobilized Recombinant Human CDK2 Protein (rp188224) at 1.0 μg/mL can bind CDK2 Mouse mAb (Ab095749) with the EC50 of 62.4 ng/mL.
Recombinant Human CDK2 Protein (rp188224) - SDS-PAGE 3 μg/lane of Recombinant Human CDK2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 33.2 kDa.