Casein Kinase 2 alpha | casein kinase 2, alpha 1 polypeptide | casein kinase II alpha 1 subunit | CK II alpha | CK2 catalytic subunit alpha | CK2A1 | CK2A1casein kinase II subunit alpha | CKII | CSNK2A1 | EC 2.7.11 | EC 2.7.11.1 | protein kinase CK2
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE),See COA
生化机理
Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via the phosphorylation of Akt.
Post-translational: Phosphorylated at Thr-344, Thr-360, Ser-362 and Ser-370 by CDK1 in prophase and metaphase and dephosphorylated during anaphase. Phosphorylation does not directly affect casein kinase 2 activity, but may contribute to its regulation by forming binding sites for interacting proteins and/or targeting it to different compartments.
生物活性
Immobilized Recombinant Human CSNK2A1 Protein (rp188235) at 1.0 μg/mL can bind Recombinant CSNK2A1 Antibody (Ab097971) with the EC50 of 5.4 ng/mL.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Casein Kinase 2 alpha Protein (rp188235) - ELISA Immobilized Recombinant Human Casein Kinase 2 alpha Protein (rp188235) at 1.0 μg/mL can bind Recombinant CSNK2A1 Antibody (Ab097971) with the EC50 of 5.4 ng/mL.
Recombinant Human Casein Kinase 2 alpha Protein (rp188235) - SDS-PAGE 3 μg/lane of Recombinant Human Casein Kinase 2 alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 43.0 kDa.
Two isoforms produced by Alternative splicing.(Uniprot:P68400)
ZJ25F0319726
危险品化学品经营许可证（带存储）