Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded β-sheet sandwiched in between a small helical amino-terminal region consisting of α1 and α2, and a large helical region comprised of α3-α10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains.
生物活性
Immobilized Recombinant Human OTUB2 Protein (rp188262) at 1.0 μg/mL can bind OTUB2 Antibody (Ab119481) with the EC50 of 301.8 ng/mL.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
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Recombinant Human OTUB2 Protein (rp188262) - ELISA Immobilized Recombinant Human OTUB2 Protein (rp188262) at 1.0 μg/mL can bind OTUB2 Antibody (Ab119481) with the EC₅₀ of 301.8 ng/mL.
Recombinant Human OTUB2 Protein (rp188262) - SDS-PAGE 3 μg/lane of Recombinant Human OTUB2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.1 kDa.