Ribonuclease Pancreatic | HP-Rnase | RIB-1 | RNase UpI-1 | Ribonuclease 1 | RNase 1 | Ribonuclease A | RNase A | RNASE1 | RIB1 | RNS1 | RAC1 | ribonuclease A family member 1 | pancreatic
规格或纯度
无动物源, 无载体, His标签, ≥95%(SDS-PAGE), expressed in HEK293; 见COA
生化机理
RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As an endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.Post-translational: N-linked glycans are of complex type.
16.6-28.8 kDa, under reducing conditions; 16.6-28.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human Ribonuclease A/RNASE1 Protein (rp188277) - SDS-PAGE
3 μg/lane of Recombinant Human Ribonuclease A/RNASE1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 16.6 & 19.9-28.8 kDa.
ZJ26F0838155
危险品化学品经营许可证（带存储）