NgR3/NgRH2 (also known as reticulon-4 receptor-Like 1), along with the related NgR1 and NgR2, is a member of the Nogo66 receptor (NgR) family. NgR proteins are expressed primarily by neurons in the central and peripheral mammalian nervous system. NgR proteins play an essential role in mediating axon growth inhibition induced by the structurally distinct proteins Nogo, myelin associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMgp). These proteins belong to the family of myelin-associated inhibitors (MAIs). NgR proteins are composed of 8 leucine-rich repeats (LRRs) flanked by N-terminal and C-terminal leucine-rich capping domains and are attached at the cell surface by a C-terminal glycosylphosphatidylinositol (GPI) anchor. Because NgR proteins have no transmembrane domain, they must be associated with membrane proteins in order to transduce cell signaling. Both NGF R/TNFRSF16 (p75) and TROY/TNFRSF19 have been identified as NgR1-associated components. Human NgR3 shares 88% and 86% amino acid sequence identity with mouse and rat NgR3 respectively. NgR1 and NgR3 serve as receptors for chondroitin sulfate proteoglycans (CSPGs). NgR3 may function as a NgR1 co-receptor for Nogo66. CSPGs and MAIs are known to stabilize neuronal structure and influence the growth and guidance of developing neurons. Therefore, NgR proteins are part of a multicomponent receptor system implicated in pathways that limit neuronal growth and increase the structural stability of synapses.
生物活性
Immobilized Recombinant Human NgR3/NgRH2 Protein (rp188278) at 1.0 μg/mL can bind Recombinant NgR3 Antibody (Ab118008) with the EC50 of 3.0 ng/mL.
52.5-66.4 kDa, under reducing conditions; 48.2-64.9 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human NgR3/NgRH2 Protein (rp188278) - ELISA Immobilized Recombinant Human NgR3/NgRH2 Protein (rp188278) at 1.0 μg/mL can bind Recombinant NgR3 Antibody (Ab118008) with the EC₅₀ of 3.0 ng/mL.
Recombinant Human NgR3/NgRH2 Protein (rp188278) - SDS-PAGE 3 μg/lane of Recombinant Human NgR3/NgRH2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 52.5-66.4 kDa under reducing conditions and 48.2-64.9 kDa under non-reducing conditions.