Immobilized Recombinant Human S100A1 Protein (rp188279) at 5.0 μg/mL can bind Recombinant Human S100B Protein (rp188279) with a linear range of 0.0007-0.56 μg/mL.
S100A1 is a Ca2+ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.
Post-translational: Glutathionylated; glutathionylation increases affinity to calcium about 10-fold.
生物活性
Immobilized Recombinant Human S100A1 Protein (rp188279) at 5.0 μg/mL can bind Recombinant Human S100B Protein (rp188279) with a linear range of 0.0007-0.56 μg/mL.
10.0 kDa, under reducing conditions; 10.0 & 20.9 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human S100A1 Protein (rp188279) - Binding Activity Immobilized Recombinant Human S100A1 Protein (rp188279) at 5.0 μg/mL can bind Recombinant Human S100B Protein (rp155671) with a linear range of 0.7-560 ng/mL.
Recombinant Human S100A1 Protein (rp188279) - SDS-PAGE 3 μg/lane of Recombinant Human S100A1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 10.0 kDa under reducing conditions and 10.0 & 20.9 kDa under non-reducing conditions. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (PubMed: 21296671).