Immobilized Recombinant Human Galectin-3 Protein (rp179829) at 2.0 μg/mL can bind Recombinant Human CEACAM-21 Protein (rp188290) with the EC50 of 1.22 μg/mL.
Carcinoembryonic antigen-related cell adhesion molecule-21 (CEACAM-21) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. The CEACAM-21 gene codes for both the CEACAM-3 and CEACAM-21 proteins and is located on chromosome 19. For CEACAM-21, the gene codes a 258 amino acid (aa) polypeptide consisting of a 206 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 32 aa cytoplasmic domain. The mature ECD contains a single IgV-like domain, common to all members of the CEACAM family, and one IgC2-like domain. CEACAM-21 is thought to be a primate-specific gene, and to date, no orthologs have been identified in lower species. Originally discovered as a biomarker for colorectal cancer, CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis. Although the specific function of the CEACAM-21 protein has yet to be elucidated, CEACAM-21 has been found to be overexpressed in heavy smokers and is associated with COPD susceptibility. It is also a candidate gene for schizophrenia in the Jewish population. CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils. Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators.
生物活性
Immobilized Recombinant Human Galectin-3 Protein (rp179829) at 2.0 μg/mL can bind Recombinant Human CEACAM-21 Protein (rp188290) with the EC50 of 1.22 μg/mL.
30.7-38.3 kDa, under reducing conditions; 29.3-36.9 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human CEACAM-21 Protein (rp188290) - Binding Activity Immobilized Recombinant Human Galectin-3 Protein (rp179829) at 2.0 μg/mL can bind Recombinant Human CEACAM-21 Protein (rp188290) with the EC50 of 1.22 μg/mL.
Recombinant Human CEACAM-21 Protein (rp188290) - SDS-PAGE 3 μg/lane of Recombinant Human CEACAM-21 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.7-38.3 kDa under reducing conditions and 29.3-36.9 kDa under non-reducing conditions.