Cysteine-rich Protein With EGF-like Domains 1 | AVSD2 | CIRRIN | JELANS | UNQ188/PRO214 | CRELD1 | Protein disulfide isomerase CRELD1 | Cysteine-rich with EGF-like domain protein 1
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,高性能,His标签,≥95%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Cysteine-rich with EGF-like domain protein 1 (CRELD1) is an approximately 50 kDa transmembrane glycoprotein that contains a 333 amino acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa. Alternative splicing of human CRELD1 generates an additional isoform with a substitution that encompasses the transmembrane segments and the C‑terminal ECD. CRELD1 is widely expressed during development and in the adult. It interacts with Reticulon 3/RTN3 and inhibits the pro‑apoptotic activity of RTN3. CRELD1 also associates with Adrenergic alpha 1 receptors in the prostate and limits their expression. Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart. R&D Systems in‑house testing indicates that CRELD1 mediates the adhesion of ATDC-5 cells.
生物活性
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1 μg/mL.
38.5-44.1 kDa; under reducing conditions; 36.7-42.4 kDa; under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human CRELD1 Protein (rp188299) - Protein Bioactivity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1 μg/mL.
Recombinant Human CRELD1 Protein (rp188299) - SDS-PAGE
3 μg/lane of Recombinant Human CRELD1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 38.5-44.1 kDa under reducing conditions and 36.7-42.4 kDa under non-reducing conditions.