级别和纯度: 无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥95%(SDS-PAGE)See COA
Accession #
P00374
蛋白标签
C-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
1. Measured by the reduction of dihydrofolic acid (DHF). The specific activity is >5,500 pmol/min/μg, as measured under the described conditions. 2. Immobilized Recombinant Human DHFR Protein (rp188305) at 1.0 μg/mL can bind Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) with the EC50 of 4.9 ng/mL.
无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE),See COA
生化机理
Dihydrofolate Reductase (DHFR) is an approximately 21 kDa enzyme that catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, which is crucial for the synthesis of purines, thymidylate, and certain amino acids. Structurally, DHFR consists of an eight-stranded beta -sheet and four alpha -helices. Human DHFR shares 90% amino acid identity with mouse and rat DHFR. In addition to acting as a cofactor, NADPH protects DHFR from degradation in vitro and in a cellular context. DHFR protein binds to its own mRNA to self-regulate translation. The enzymatic activity and subcellular localization of DHFR are at least partially regulated by post-translational modification. Mono-ubiquitination of DHFR by MDM2 reduces the activity of DHFR. DHFR is primarily cytoplasmic, but it has been shown to translocate to the nucleus in a SUMO-dependent manner. Due to its role in nucleotide biosynthesis, DHFR has long been a therapeutic target for the treatment of various cancers and infectious diseases.
生物活性
1. Measured by the reduction of dihydrofolic acid (DHF). The specific activity is >5,500 pmol/min/μg, as measured under the described conditions. 2. Immobilized Recombinant Human DHFR Protein (rp188305) at 1.0 μg/mL can bind Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) with the EC50 of 4.9 ng/mL.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human DHFR Protein (rp188305) - ELISA Immobilized Recombinant Human DHFR Protein (rp188305) at 1.0 μg/mL can bind Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) with the EC50 of 4.9 ng/mL.
Recombinant Human DHFR Protein (rp188305) - SDS-PAGE 2 μg/lane of Recombinant Human DHFR Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 23.5 kDa.
Recombinant Human DHFR Protein - Enzyme Activity Measured by the reduction of dihydrofolic acid (DHF). The specific activity is >5,500 pmol/min/μg, as measured under the described conditions. Materials: Assay Buffer: 50 mM MES, 25 mM Tris, 100 mM NaCl, 25 mM Ethanolamine, 2 mM DTT Recombinant Human DHFR Protein (rp188305Z) beta -Nicotinamide adenine dinucleotide phosphate reduced, tetrasodium salt ( beta -NADPH) (N276326), 10 mM stock in deionized water Dihydrofolic acid (DHF) (D135507), 10 mM stock in Assay Buffer + 4.5 mM NaOH Procedures： 1.Dilute rhDHFR to 0.33 μg/mL in Assay Buffer. 2. Prepare a Substrate Mixture containing 0.8 mM DHF and 1 mM beta -NADPH in Assay Buffer. 3. Load 50 μL of 0.33 μg/mL rhDHFR into a plate, and start the reaction by adding 50 μL of Substrate Mixture. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of Substrate Mixture. 4. Read at an absorbance of 339 nm in kinetic mode for 5 minutes. 5. Calculate specific activity: *Adjusted for Substrate Blank
**Using the extinction coefficient 6220 M⁻¹cm⁻¹
***Using the path correction 0.32 cmNote: the output of many spectrophotometers is in mOD. Per Well: rhDHFR: 0.0165 μg, DHF: 0.4 mM, beta -NADPH: 0.5 mM
ZJ25F0218478
危险品化学品经营许可证（带存储）