无动物源,无载体,His标签,≥95%(SDS-PAGE),expressed in HEK293, 见 COA
生化机理
DMBT1 (Deleted in Malignant Brain Tumor 1) also known as Glycoprotein 340 and salivary agglutinin is a secreted glycoprotein with mass of 340 kDa. It belongs to group B of the SRCR (scavenger cysteine-rich) superfamily with each SRCR domain containing 8 cysteines. Primary sequence of DMBT1 is composed of 8-13 SRCR domains (due to alternative splicing), a CUB domain, another SRCR domain, a second CUB domain and ended with a ZP (zona pellucida) domain. DMBT1 is heavily glycosylated where N-linked and O-linked glycosylation account for almost 25% of the mass. It is mainly expressed in epithelial cells and mucosal tissues of respiratory and gastrointestinal tracts and urogenital organs. It is also found in human body fluids (saliva, tear, broncho-alveolar lavage, and pancreatic juice). Extent of glycosylation varies among different tissues or individuals. Orthologues of DMBT1 have been found in monkey, mouse, rat, rabbit, bovine, and porcine. Human DMBT1 shares 49% and 35% amino acid homology with mouse and rat homolog. DMBT1 plays an important role in innate immune response. It is an agglutinating agent for several bacteria strains through a specific region in the SRCR domain in a calcium dependent way. It can also bind to viruses such as influenza A virus and human immunodeficiency virus type I and inhibit their activity. Binding to viruses is mediated by the sialic acid glycan of DMBT-1, independent of calcium. In addition to binding pathogens, DMBT-1 also binds a broad spectrum of host proteins including SP-d, SP-A, secretory IgA, refoil factors (TFFs), MUC5B, complement factor C1q, lactoferrin and DNA. Recent studies identify DMBT1 as a high affinity ligand of Siglec-8 in human airways. Inactivation of DMBT1 affects stem cell differentiation and results in tumor formation.
42.1-51.3 kDa, under reducing conditions; 42.1-51.3 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293, 见 COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human DMBT1 Protein (rp188308) - SDS-PAGE
3 μg/lane of Recombinant Human DMBT1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 42.1-51.3 kDa.
ZJ26F0333116
危险品化学品经营许可证（带存储）