Desmocollin-3 is a 100 - 110 kDa type I transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules. The human Desmocollin-3 is synthesized with a 108 amino acid (aa) propeptide. The mature protein consists of a 555 aa extracellular domain (ECD) that contains five cadherin-like domains, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain. Within the ECD, human Desmocollin-3 shares 78% aa sequence identity with mouse and rat Desmocollin-3. It shares 52% and 64% aa sequence identity with Desmocollin-1 and -2, respectively. A splice variant of human Desmocollin-3 has a truncated and substituted cytoplasmic domain. Desmocollin-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells. It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues. Desmocollin-1 and -2, by contrast, are preferentially localized in the more superficial layers. In mouse, Desmocollin-3 is expressed throughout embryogenesis and is required for non-desmosomal functions during early development. During apoptosis, both isoforms of Desmocollin-3 are proteolytically cleaved by caspases and metalloproteases. Desmocollin-3 can be upregulated or downregulated in carcinoma.
72.4 kDa, under reducing conditions; 76.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Desmocollin-3 Protein (rp188311) - SDS-PAGE 3 μg/lane of Recombinant Human Desmocollin-3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 72.4 kDa under reducing conditions and 76.0 kDa under non-reducing conditions.
ZJ25F0420786
危险品化学品经营许可证（带存储）