Anticoagulant protein C | APC | Autoprothrombin IIA | Blood coagulation factor XIV | Coagulation Factor XIV | EC 3.4.21 | EC 3.4.21.69 | PC | PROC | PROC1 | protein C (inactivator of coagulation factors Va and VIIIa) | Protein C | vitamin K-dependent prot
规格或纯度
无动物源,无载体,His标签,PBS Only,≥90%(SDS-PAGE),见COA
生化机理
PROC (protein C gene) is a 62-kDa vitamin K-dependent plasma zymogen which, after activation to serine protease, plays an important role in the physiologic regulation of blood coagulation. Originally thought to be synthesized exclusively by the liver, recent reports have shown that PROC is also produced by many other cells including pancreatic islet β cells. Protein C a vitamin K-dependent plasma glycoprotein and is one of the body’s natural anticoagulants circulating at 70 nmol/L as an inactive protease zymogen.
Post-translational: The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium. N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
52.0-68.1 kDa, under reducing conditions; 52.0-68.1 kDa, under non-reducing conditions.
分子类型
蛋白质
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human Coagulation Factor XIV/Protein C Protein (rp188323) - SDS-PAGE 3 μg/lane of Recombinant Human Coagulation Factor XIV/Protein C Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 52.0-68.1 kDa under reducing conditions and non-reducing conditions. Synthesized as a single chain precursor, which is cleaved into a light chain and a heavy chain held together by a disulfide bond. The enzyme is then activated by thrombin, which cleaves a tetradecapeptide from the amino end of the heavy chain; this reaction, which occurs at the surface of endothelial cells, is strongly promoted by thrombomodulin. Interacts (activated) with iripin-8, a serine protease inhibitor from Ixodes ricinus saliva (PubMed:34502392;Unirot:P04070).
ZJ25F1028823
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