CD148 antigen | CD148 | Density-enhanced phosphatase 1 | DEP1 | DEP-1 | DEP1EC 3.1.3.48 | HPTP eta | HPTPeta | HPTP-eta | human density enhanced phosphatase-1 | protein tyrosine phosphatase | Protein-tyrosine phosphatase eta | Protein-tyrosine phosphatase
规格或纯度
无载体,His标签,PBS Only,≥95%(SDS-PAGE)
生化机理
Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density. DEP-1 phosphatase activity is enhanced by basement membrane proteins, suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF, VEGF, and T-cell receptor responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers. In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes. DEP-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas. Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development. DEP-1 dephosphorylates a variety of proteins, including the HGF, PDGF, and VEGF receptors, and beta‑catenin. The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.
Post-translational: N- and O-glycosylated.
36.9 kDa, under reducing conditions; 34.8 & 37.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.5.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human DEP-1/CD148 Protein (rp188326) - SDS-PAGE 3 μg/lane of Recombinant Human DEP-1/CD148 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 36.9 kDa under reducing conditions and 34.8 & 37.2 kDa under non-reducing conditions.
ZJ25F0217503
危险品化学品经营许可证（带存储）