GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
Post-translational: (Microbial infection) Demyristoylated by S.flexneri cysteine protease IpaJ which cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3.
生物活性
Immobilized Recombinant Human ARF1 Protein (rp188332) at 1.0 μg/mL can bind Recombinant ARF1 Antibody (Ab089466) with the EC50 of 4.8 ng/mL.
18.0 kDa, under reducing conditions; 18.4 & 38.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human ARF1 Protein (rp188332) - ELISA Immobilized Recombinant Human ARF1 Protein (rp188332) at 1.0 μg/mL can bind Recombinant ARF1 Antibody (Ab089466) with the EC₅₀ of 4.8 ng/mL.
Recombinant Human ARF1 Protein (rp188332) - SDS-PAGE 3 μg/lane of Recombinant Human ARF1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 18.0 kDa under reducing conditions and 18.4 & 38.0 kDa under non-reducing conditions. ARF1 dimerization is essential for vesicle trafficking and dependent on activation by ARF-GEF dimers in Arabidopsis (PMID: 32487565).