C SRC | C SRC kinase | C src Tyrosine Kinase | C-SRC kinase | c-src tyrosine kinase | CSK | Csk A | CSK_HUMAN | CYTOPLASMIC TYROSINE KINASE | EC 2.7.10.2 | MGC112926 | MGC117393 | MGC154049 | P60 Src | Protein tyrosine kinase CYL | Protein-tyrosine kinase
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥90%(SDS-PAGE),见COA
生化机理
Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
Post-translational: Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated.
生物活性
Immobilized Recombinant Human CSK Protein (rp188360) at 1.0 μg/mL can bind Recombinant CSK Antibody (Ab097931) with the EC50 of 6.4 ng/mL.
50.8 kDa, under reducing conditions; 50.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 20% Glycerol, 1 mM TCEP, pH 7.4
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃ 存储6个月;收货后建议分装,避免反复冻融。
图片
Recombinant Human CSK Protein (rp188360) - ELISA Immobilized Recombinant Human CSK Protein (rp188360) at 1.0 μg/mL can bind Recombinant CSK Antibody (Ab097931) with the EC50 of 6.4 ng/mL.
Recombinant Human CSK Protein (rp188360) - SDS-PAGE 3 μg/lane of Recombinant Human CSK Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 50.8 kDa.