Recombinant Human GRB2 Protein

货号: rp188402
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 ≥90%(SDS-PAGE) 见COA
Accession #
P62993
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human GRB2 Protein (rp188402) at 1.0 μg/mL can bind GRB2 Mouse mAb (Ab106387) with the EC50 of 30.5 ng/mL.
★
规格
库存
价格
数量
10μg
rp188402-10μg
现货 Stock Image
¥819.90
50μg
rp188402-50μg
现货 Stock Image
¥2,449.90
100μg
rp188402-100μg
现货 Stock Image
¥3,899.90
1mg
rp188402-1mg
现货 Stock Image
¥19,999.90
请输入您要添加的产品数量
🧪
为什么选择此级别

ActiBioPure™,His标签,无载体,生物活性 级 提供 ≥90%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Human GRB2 Protein
别名
重组人GRB2蛋白 | 重组人生长因子受体结合蛋白2
英文别名
Abundant SRC homology | Adapter protein GRB2 | ASH | Ash protein | EGFRBP GRB2 | Epidermal growth factor receptor binding protein | Epidermal growth factor receptor binding protein GRB2 | GRB 2 | Grb2 | GRB2 adapter protein | GRB2_HUMAN | Grb3 3 | Growth
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥90%(SDS-PAGE),见COA
生化机理
GRB2 is widely expressed and binds the EGFR and contains one SH2 domain and two SH3 domains which form a complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. GRB2 is related to the Sem5 gene of C.elegans, which is takes part in the signal transduction pathway. GRB2 is an adaptor protein that provides an important link between cell surface growth factor receptors and the Ras signaling pathway. Inhibition of GRB2 activity damages developmental processes in a variety of organisms and blocks transformation and proliferation of different cell types. Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. Post-translational: Phosphorylation of Tyr-209 in the C-terminal SH3 domain reduces its binding to SOS1. Ubiquitinated by RNF173, leading to proteasomal degradation and inhibition of the RAF/MEK/ERK pathway (PubMed:37328606). In the nucleus, polyubiquitinated by RBBP6 at Lys-109 at DNA damage sites (PubMed:34348893).
生物活性
Immobilized Recombinant Human GRB2 Protein (rp188402) at 1.0 μg/mL can bind GRB2 Mouse mAb (Ab106387) with the EC50 of 30.5 ng/mL.
表达系统
E. coli
种属
人(Human)
氨基酸
2-217 aa
序列
MHHHHHHEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV
蛋白标签
N-His
无载体
Yes
无动物源
No
来源
重组表达
预测分子量
26.0 kDa
SDS-PAGE
26.3 kDa, under reducing conditions; 24.6 & 26.7 & 49.3 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
20mM Tris, 100mM NaCl, 30% Glycerol, pH 8.0
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片

Recombinant Human GRB2 Protein (rp188402) - ELISA
Immobilized Recombinant Human GRB2 Protein (rp188402) at 1.0 μg/mL can bind GRB2 Mouse mAb (Ab106387) with the EC₅₀ of 30.5 ng/mL.

Recombinant Human GRB2 Protein (rp188402) - SDS-PAGE
3 μg/lane of Recombinant Human GRB2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 26.3 kDa under reducing conditions and 24.6 & 26.7 & 49.3 kDa under non-reducing conditions.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到4个结果

批号(Lot Number) 证书类型 货号
ZJ24F1216002 分析证书 rp188402
ZJ24F1216003 分析证书 rp188402
ZJ24F1216004 分析证书 rp188402
ZJ24F1216005 分析证书 rp188402
溶液计算器