Immobilized Recombinant Human Glutathione Synthetase Protein (rp188403) at 1.0 μg/mL can bind Recombinant Glutathione Synthetase Antibody (Ab105758) with the EC50 of 7.1 ng/mL.
Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner (PubMed:7646467, PubMed:9215686). Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes (PubMed:10369661). Participates in ophthalmate biosynthesis in hepatocytes (By similarity).
生物活性
Immobilized Recombinant Human Glutathione Synthetase Protein (rp188403) at 1.0 μg/mL can bind Recombinant Glutathione Synthetase Antibody (Ab105758) with the EC50 of 7.1 ng/mL.
49.6 kDa, under reducing conditions; 49.6 & 49.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM Tris, 200mM NaCl, pH 7.5
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human Glutathione Synthetase Protein (rp188403) - ELISA Immobilized Recombinant Human Glutathione Synthetase Protein (rp188403) at 1.0 μg/mL can bind Recombinant Glutathione Synthetase Antibody (Ab105758) with the EC50 of 7.1 ng/mL.
Recombinant Human Glutathione Synthetase Protein (rp188403)- SDS-PAGE 3 μg/lane of Recombinant Human Glutathione Synthetase Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 49.6 kDa under reducing conditions and 45.5 & 49.8 kDa under non-reducing conditions. The presence of two bands under non-reducing conditions may be due to the protein existing in two different conformations in this state (Uniprot: P48637)
ZJ25F0926722
危险品化学品经营许可证（带存储）