Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.
生物活性
Immobilized Recombinant Human Gankyrin Protein (rp188418) at 1.0 μg/mL can bind Gankyrin Mouse mAb (Ab104742) with the EC50 of 59.5 ng/mL.
24.3 kDa, under reducing conditions; 24.9 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 10% Glycerol, pH 7.4
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Gankyrin Protein (rp188418) - ELISA Immobilized Recombinant Human Gankyrin Protein (rp188418) at 1.0 μg/mL can bind Gankyrin Mouse mAb (Ab104742) with the EC₅₀ of 59.5 ng/mL.
Recombinant Human Gankyrin Protein (rp188418) - SDS-PAGE 3 μg/lane of Recombinant Human Gankyrin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 24.3 kDa under reducing conditions and 24.9 kDa under non-reducing conditions.