Ras-related protein Rab-27B | C25KG | RAB27B | member RAS oncogene family
规格或纯度
无动物源, 无载体, 生物活性, ActiBioPure™, His标签, ≥95%(SDS-PAGE), expressed in HEK293; 见COA
生化机理
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:21775604, PubMed:30771381).
RAB27B regulates homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed:30771381).
Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 (PubMed:21775604).
May be involved in targeting uroplakins to urothelial apical membranes (By similarity).
生物活性
Immobilized Recombinant Human RAB27B Protein (rp188424) at 1.0 μg/mL can bind RAB27B Mouse mAb (Ab124166) with the EC50 of 13.08 ng/mL.
26.9 kDa, under reducing conditions; 25.1 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human RAB27B Protein (rp188424) - ELISA
Immobilized Recombinant Human RAB27B Protein (rp188424) at 1.0 μg/mL can bind RAB27B Mouse mAb (Ab124166) with the EC50 of 13.08 ng/mL.
Recombinant Human RAB27B Protein (rp188424) - SDS-PAGE
3 μg/lane of Recombinant Human RAB27B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band 26.9 kDa under reducing conditions and 25.1 kDa under non-reducing conditions.