计算溶液所需的质量、体积或浓度。
Recombinant Human Calsyntenin-2 Protein
有货
为什么选择此级别
His标签,无动物源,无载体 级 提供 ≥90%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
规格
产品名称
Recombinant Human Calsyntenin-2 Protein
别名
重组人Calsyntenin-2蛋白 | 重组人钙结合突触蛋白2
英文别名
alcadein gamma | Alcadein-gamma | alcagamma | ALC-GAMMA | cadherin-related family member 13 | calsyntenin 2 | Calsyntenin2 | Calsyntenin-2 | CDHR13 | CLSTN2 | CS2 | CS2alcadein-gamma | CSTN2 | FLJ39113 | FLJ39499 | MGC119560
规格或纯度
无动物源,无载体,His标签,≥90%(SDS-PAGE),见COA
生化机理
Calsyntenin-2, also called Alc-gamma (Alcadein-gamma; gene name CLSTN2) is a 120-135 kDa glycoprotein that is a member of the Alcadein family of cadherin domain‑containing type I transmembrane molecules. Mature human Calsyntenin-2 is a 935 amino acid (aa) protein with two extracellular cadherin domains. Proteolytic cleavage by ADAM10 and gamma -secretase generates a 105-110 kDa extracellular domain termed soluble alc-gamma, and a 25 kDa, highly acidic, calcium-binding intracellular domain termed Alc gamma ICD. The soluble alc-gamma portion of human Calsyntenin-2 (aa 21-831) shares 95%, 94%, 96%, 95% and 94% aa identity with mouse, rat, canine, bovine and equine Calsyntenin-2, respectively. A potential isoform variant shows a premature truncation after Gln608. Calsyntenins are predominantly expressed in the ER/Golgi and post-synaptic plasma membranes of neurons. While nearly all neurons express Calsyntenin 1, Calsyntenin-2 is mainly found in subpopulations GABAergic neurons of cortical layers 5 and 6, cerebellum (in Purkinje cells), amygdale (basolateral cells), hippocampus (pyramidal cells and hilus interneurons) and some brain stem neurons. Calsyntenin-2 interacts with amyloid precursor protein (APP) through its binding protein, APBA2/X11L, where it is thought to regulate APP cleavage, and likely impacts post-synaptic signaling and gene activation. Notably, Alc gamma ICD binds APBA1/FE65 and antagonizes amyloid intracellular domain (AICD) gene activation. Polymorphisms of the Calsyntenin-2 gene, in combination with polymorphisms of the brain signaling regulator KIBRA, are thought to modulate episodic memory performance.
Post-translational: Proteolytically processed under normal cellular conditions (PubMed:15037614). A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1) (PubMed:15037614). A secondary cleavage catalyzed by gamma-secretase within the transmembrane domain releases the beta-Alc-gamma chain in the extracellular milieu and produces an intracellular fragment (AlcICD) (PubMed:15037614). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1 (PubMed:15037614).
表达系统
HEK293
种属
人(Human)
氨基酸
1-831 aa
序列
MLPGRLCWVPLLLALGVGSGSGGGGDSRQRRLLAAKVNKHKPWIETSYHGVITENNDTVILDPPLVALDKDAPVPFAGEICAFKIHGQELPFEAVVLNKTSGEGRLRAKSPIDCELQKEYTFIIQAYDCGAGPHETAWKKSHKAVVHIQVKDVNEFAPTFKEPAYKAVVTEGKIYDSILQVEAIDEDCSPQYSQICNYEIVTTDVPFAIDRNGNIRNTEKLSYDKQHQYEILVTAYDCGQKPAAQDTLVQVDVKPVCKPGWQDWTKRIEYQPGSGSMPLFPSIHLETCDGAVSSLQIVTELQTNYIGKGCDRETYSEKSLQKLCGASSGIIDLLPSPSAATNWTAGLLVDSSEMIFKFDGRQGAKVPDGIVPKNLTDQFTITMWMKHGPSPGVRAEKETILCNSDKTEMNRHHYALYVHNCRLVFLLRKDFDQADTFRPAEFHWKLDQICDKEWHYYVINVEFPVVTLYMDGATYEPYLVTNDWPIHPSHIAMQLTVGACWQGGEVTKPQFAQFFHGSLASLTIRPGKMESQKVISCLQACKEGLDINSLESLGQGIKYHFNPSQSILVMEGDDIGNINRALQKVSYINSRQFPTAGVRRLKVSSKVQCFGEDVCISIPEVDAYVMVLQAIEPRITLRGTDHFWRPAAQFESARGVTLFPDIKIVSTFAKTEAPGDVKTTDPKSEVLEEMLHNLDFCDILVIGGDLDPRQECLELNHSELHQRHLDATNSTAGYSIYGVGSMSRYEQVLHHIRYRNWRPASLEARRFRIKCSELNGRYTSNEFNLEVSILHEDQVSDKEHVNHLIVQPPFLQSVHHPESRSSIQHSSVVPSHHHHHH
蛋白标签
C-His
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
92.0 kDa
SDS-PAGE
97.5-120.1 kDa, under reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 6 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？如何测定？
本产品的纯度为 ≥90%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。
什么是「无动物源」？
「无动物源」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。
什么是「无载体」？
「无载体」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。
本产品应如何储存？
请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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