Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.
生物活性
Immobilized Recombinant Human UAP56 Protein (rp188494) at 1.0 μg/mL can bind UAP56 Antibodydy (Ab133187) with the EC50 of 48.8 ng/mL.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
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Recombinant Human UAP56 Protein (rp188494) - ELISA Immobilized Recombinant Human UAP56 Protein (rp188494) at 1.0 μg/mL can bind UAP56 Antibodydy (Ab133187) with the EC50 of 48.8 ng/mL.
Recombinant Human UAP56 Protein (rp188494) - SDS-PAGE 3 μg/lane of Recombinant Human UAP56 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 49.1 kDa.
ZJ25F0420685
危险品化学品经营许可证（带存储）