CBX 3 | CBX3 | CBX3_HUMAN | Chromobox homolog 3 | Chromobox homolog 3 (HP1 gamma homolog, Drosophila) | Chromobox protein homolog 3 | GAMMA | HECH | Heterochromatin like protein 1 | Heterochromatin protein 1 homolog gamma | Heterochromatin protein HP1 gam
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥90%(SDS-PAGE),见COA
生化机理
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).
Post-translational: Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
生物活性
Immobilized Recombinant Human HP1 gamma/CBX3 Protein (rp189288) at 1.0 μg/mL can bind HP1 gamma/CBX3 Antibody (Ab108619) with the EC50 of 51.8 ng/mL.
Recombinant Human HP1 gamma/CBX3 Protein (rp189288) - ELISA Immobilized Recombinant Human HP1 gamma/CBX3 Protein (rp189288) at 1.0 μg/mL can bind HP1 gamma/CBX3 Antibody (Ab108619) with the EC50 of 51.8 ng/mL.
Recombinant Human HP1 gamma/CBX3 Protein (rp189288) - SDS-PAGE 3 μg/lane of Recombinant Human HP1 gamma/CBX3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 22.2 & 45.4 kDa under reducing conditions and 22.7 & 44.6 & 67.8 kDa under non-reducing conditions. HP1 gamma/CBX3 forms polymers through intermolecular disulfide bonds of cysteine in the hinge region (PMID: 12805230).