Cytoplasmic Polyadenylation Element Binding Protein 1 | CPE-Binding Protein 1 | CPE-BP1 | HCPEB-1 | CPEB | Cytoplasmic Polyadenylation Element-Binding Protein 1 | CPEB-1 | H-CEBP | CEBP | CPEB1
规格或纯度
无载体, 生物活性, ActiBioPure™, His标签, ≥80%(SDS-PAGE), expressed in E. coli; 见COA
生化机理
Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967).
In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity).
Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity).
Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity).
Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry (PubMed:26398195).
Post-translational: Phosphorylated on serine/threonine residues by AURKA within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA (By similarity).
生物活性
Immobilized Recombinant Human CPEB1 Protein (rp189330) at 1.0 μg/mL can bind Recombinant CPEB1 Antibody (Ab097517) with the EC50 of 13.34-15.61 ng/mL.
Recombinant Human CPEB1 Protein (rp189330)- Binding Activity
Immobilized Recombinant Human CPEB1 Protein (rp189330) at 1.0 μg/mL can bind Recombinant CPEB1 Antibody (Ab097517) with the EC50 of 13.34-15.61 n ng/mL.
Recombinant Human CPEB1 Protein (rp189330) - SDS-PAGE
3 μg/lane of Recombinant Human CPEB1 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 60.9 kDa under reducing conditions.
CPEB1 protein contains a glutamine-rich (Q-rich) domain at its N-terminus. This domain has an inherent ability to bind to itself independently of specific sequences, promoting the aggregation of CPEB1 molecules (Uniprot ID: Q9BZB8)
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