级别和纯度: 无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥85%(SDS-PAGE)expressed in E. coli; 见COA
无载体,His标签,≥85%(SDS-PAGE),expressed in E. coli; 见COA
生化机理
DNAJB11 is a member of the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJB11 serves as a co-chaperone for HSPA5 and binds directly to both unfolded proteins which are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed.
Post-translational: Contains high-mannose Endo H-sensitive carbohydrates. Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known. Thr-188 was reported to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.
Recombinant Human DNAJB11 Protein (rp189392) - SDS-PAGE
2 μg/lane of Recombinant Human DNAJB11 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 39.8 kDa under reducing conditions and 38.4 & 39.8 kDa under non-reducing conditions.
The formation of intramolecular disulfide bonds among Cys-169, Cys-171, Cys-193, and Cys-196 in DNAJB11 could explain the presence of closely migrating isomers (Uniprot ID: Q9UBS4).
ZJ26F0434608
危险品化学品经营许可证（带存储）