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Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423) - Binding Activity
Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp189423) with the EC50 of 25.02 ng/mL.
ActiBioPure™,His标签,无载体,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Protein Function:
CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region. The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms. The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable. CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells. The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.
Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423) - Binding Activity
Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp189423) with the EC50 of 25.02 ng/mL.
Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423) - Binding Activity
Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp189423) with the EC₅₀ of 25.02 ng/mL.
Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423) - ELISA
Immobilized Recombinant Human CD40 Ligand/TNFSF5 protein (rp189423) at 1.0 μg/mL can bind Dapirolizumab (anti-CD40L) (Ab191960) with the EC₅₀ of 28.88 ng/mL.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp189423 | |
| 分析证书 | rp189423 | |
| 分析证书 | rp189423 |