B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. Mature human B7-1 consists of a 208 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Within the ECD, human B7-1 shares 50% aa sequence identity with mouse B7-1. Alternative splicing generates a 30 kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment. Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.
Post-translational: Palmitoylated by ZDHHC20; palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization.
生物活性
Immobilized Recombinant Human CD80 Protein (rp189426) at 1.0 μg/mL can bind Galiximab (anti-CD80) (Ab177850) with the EC50 of 95.6 ng/mL.
39.2-48.6 kDa, under reducing conditions; 38.5-47.5 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human B7-1/CD80 Protein (rp189426) - ELISA Immobilized Recombinant Human B7-1/CD80 Protein (rp189426) at 1.0 μg/mL can bind Galiximab (anti-CD80) (Ab177850) with the EC50 of 95.6 ng/mL.
Recombinant Human B7-1/CD80 Protein (rp189426) - SDS-PAGE 3 μg/lane of Recombinant Human B7-1/CD80 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 39.2-48.6 kDa under reducing conditions and 38.5-47.5 kDa under non-reducing conditions.