Recombinant Human Proteasome subunit beta type 2/PSMB2 Protein
别名
重组人Proteasome subunit beta type 2/PSMB2蛋白 | 重组人蛋白酶体20S亚基β 2蛋白
英文别名
HC7 I | Macropain subunit C7 I | Macropain subunit C7-I | Multicatalytic endopeptidase complex subunit C7 1 | Multicatalytic endopeptidase complex subunit C7 I | Multicatalytic endopeptidase complex subunit C7-I | Proteasome (prosome, macropain) subunit b
规格或纯度
无载体,His标签,≥90%(SDS-PAGE),见COA
生化机理
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 6个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human Proteasome subunit beta type 2/PSMB2 Protein (rp189463) - SDS-PAGE 2 μg/lane of Recombinant Human Proteasome subunit beta type 2/PSMB2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 21.5 kDa under reducing conditions and 20.7 & 38.3 & 40.3 kDa under non-reducing conditions. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7 (PMID：25599644).
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