Phosphoglycerate kinase 1, also referred to PGK1, is an X-linked enzyme whichfunctions in the glycolytic pathway.The gene encoding the erythrocyte enzyme PGK1 & it seems that PGK-1 plays a role as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP.
Post-translational: Phosphorylated at Ser-203 by MAPK1/ERK2 under hypoxic conditions, which promotes its mitochondrial targeting.
生物活性
Immobilized Recombinant Human PGK-1 Protein (rp189488) at 1.0 μg/mL can bind PGK1 Antibody (Ab121480) with the EC50 of 47.5 ng/mL.
42.0 kDa, under reducing conditions; 42.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 10% Glycerol, pH 7.4.
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃(6个月);收货后建议分装,避免反复冻融。
图片
Recombinant Human PGK-1 Protein (rp189488) - ELISA
Immobilized Recombinant Human PGK-1 Protein (rp189488) at 1.0 μg/mL can bind PGK1 Antibody (Ab121480) with the EC₅₀ of 47.5 ng/mL.
Recombinant Human PGK-1 Protein (rp189488) - SDS-PAGE
3 μg/lane of Recombinant Human PGK-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 42.0 kDa.