Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels (PubMed:20231281, PubMed:23426360, PubMed:29765047, PubMed:31695197). Is involved in adipocyte differentiation (By similarity). Recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity). Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression (By similarity).
Post-translational: Phosphorylated at multiple sites by mTOR in response to insulin, leading to its inactivation (PubMed:23426360). Phosphorylation does not affect the catalytic activity but regulates the localization (By similarity). Phosphorylation is decreased by epinephrine (By similarity). Dephosphorylated by the CTDNEP1-CNEP1R1 complex (By similarity). Dephosphorylation following mTOR inhibition promotes its activity (PubMed:31695197). Acetylation at Lys-425 and Lys-595 by KAT5 in response to fatty acids promotes translocation to the endoplasmic reticulum and synthesis of diacylglycerol. Sumoylated.
21.1 kDa, under reducing conditions; 44.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM Tris, 500mM NaCl, 500mM Imidazole, pH 8.0.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human Lipin 1 Protein (rp189493) - SDS-PAGE 2 μg/lane of Recombinant Human Lipin 1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 21.1 kDa under reducing conditions and 44.4 kDa under non-reducing conditions. The reason for the formation of protein dimer may be related to the following: protein interaction in lipid metabolism, on the other hand, it may also be related to protein cross-linking induced by free radicals (Uniprot: P43403).
ZJ25F0623430
危险品化学品经营许可证（带存储）