Desmin is a cytoplasmic protein and belongs to the intermediate filament family. interacts with DST and MTM1. Desmin is only expressed in vertebrates, however homologous proteins are found in many organisms. Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. DES founctions as homopolymers to form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. .Defects in DES are cause of the myopathy myofibrillar type 1, cardiomyopathy dilated type 1I, and neurogenic scapuloperoneal syndrome Kaeser type.
Post-translational: ADP-ribosylation prevents ability to form intermediate filaments. Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis. Ubiquitination by a SCF-like complex containing ASB2 isoform 1 leads to proteasomal degradation.
生物活性
Immobilized Recombinant Human Desmin Protein (rp189498) at 1.0 μg/mL can bind Desmin Antibody (Ab099656) with the EC50 of 196.3 ng/mL.
26.9 kDa, under reducing conditions; 27.3 & 56.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human Desmin Protein (rp189498) - ELISA Immobilized Recombinant Human Desmin Protein (rp189498) at 1.0 μg/mL can bind Desmin Antibody (Ab099656) with the EC50 of 196.3 ng/mL.
Recombinant Human Desmin Protein (rp189498) - SDS-PAGE 3 μg/lane of Recombinant Human Desmin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 26.9 kDa under reducing conditions and 27.3 & 56.0 kDa under non-reducing conditions. Desmin protein can form homodimers under certain conditions (PubMed: 21135508).