CCNB1, also known as cyclin B1, is a regulatory protein involved in mitosis. This protein complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Recombinant human CCNB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Post-translational: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132).Deubiquitinated by USP22 during G2/M phase (PubMed:27030811). Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
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Recombinant Human Cyclin B1 Protein (rp192056) - SDS-PAGE 3 μg/lane of Recombinant Human Cyclin B1 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 53.7 & 144.6 kDa under reducing conditions. The Cyclin B1 Protein forms dimers with various proteins, and a small amount of homodimers may be related to this (Uniprot: P14635).
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