GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor), is a 587 aa, 7-transmembrane receptor for the neuroprotective and glioprotective factor prosaposin. It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus. It is a substrate of the E3 ubiquitin ligase, parkin, which is up-regulated during endoplasmic reticulum stress. In a juvenile form of Parkinson's disease, GPR37 accumulates, contributing to stress-induced neuronal cell death. The extracellular domains (aa 27-265) of human and mouse GPR37 share 68% aa identity.
Post-translational: The N-terminus is cleaved by ADAM10 metalloproteinase; mediating limited proteolysis leading to the release of receptor ectodomain by shedding (PubMed:26869225, PubMed:34042202).In addition, cleaved by FURIN between Arg-54 and Asp-55 (PubMed:34042202). Ubiquitinated by PRKN in the presence of UBE2E1 and UBE2L3 in the endoplasmic reticulum. The unfolded form is specifically ubiquitinated by SYVN1, which promotes its proteasomal degradation and prevents neuronal cell death.
生物活性
Immobilized Recombinant Human GPR37 Protein (rp192118) at 1.0 μg/mL can bind Recombinant GPCR/GPR37 Antibody (Ab106130) with the EC50 of 2.9 ng/mL.
31.3-53.3 kDa, under reducing conditions; 31.3-53.3 kDa, under non-reducing conditions.
分子类型
蛋白质
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃(1-2周);长期储存-20℃(12个月);收货后建议分装,避免反复冻融。
图片
Recombinant Human GPR37 Protein (rp192118) - ELISA Immobilized Recombinant Human GPR37 Protein (rp192118) at 1.0 μg/mL can bind Recombinant GPCR/GPR37 Antibody (Ab106130) with the EC50 of 2.9 ng/mL.
Recombinant Human GPR37 Protein (rp192118) - SDS-PAGE 3 μg/lane of Recombinant Human GPR37 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 31.3-53.3 kDa.