94 kDa glucose regulated protein | 94 kDa glucose-regulated protein | ECGP | Endoplasmin | Endothelial cell (HBMEC) glycoprotein | ENPL_HUMAN | Glucose regulated protein 94kDa | gp96 | gp96 homolog | GRP94 | GRP 94 | GRP-94 | Heat shock protein 90 kDa bet
规格或纯度
无动物源, 无载体, His标签, ≥90%(SDS-PAGE), 见COA
生化机理
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Post-translational: Phosphorylated by CK2. N-glycosylated cotranslationally at Asn-217 by STT3A-containing OST-A complex: this glycosylation is constitutive (PubMed:31296534, PubMed:39509507). In response to various stress, 5 additional facultative sites (Asn-62, Asn-107, Asn-445, Asn-481 and Asn-502) can be glycosylated post-translationally by STT3B-containing OST-B complex, leading to a hyperglycosylated form that is degraded by the ER-associated degradation (ERAD) pathway (PubMed:31296534, PubMed:39509507). In normal conditions, the OST-A complex together with CCDC134 prevent glycosylation at facultative sites during protein folding, thereby preventing hyperglycosylation (PubMed:39509507). Mechanistically, nascent HSP90B1 is tethered during translation to a specialized CCDC134-containing translocon that forms a microenvironment for its folding, in which STT3A associates with the SRT pseudosubstrate motif, and prevents access to facultative glycosylation sites until folding is completed, rendering its facultative sites inaccessible to the OST-B complex (PubMed:39509507).
93.8 kDa, under reducing conditions; 95.8 & 200.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 1% Sucrose, 4% Mannitol, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human GRP94 Protein (rp192129) - SDS-PAGE 3 μg/lane of Recombinant Human GRP94 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 93.8 kDa under reducing conditions and 95.8 & 200.0 kDa under non-reducing conditions. There are multiple pairs of disulfide bonds in the protein sequence. Disulfide bonds cause the protein to present as a polymer in a non-reducing conditions (Uniprot ID：P14625).
ZJ26F0737369
危险品化学品经营许可证（带存储）