级别和纯度: 无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥95%(SDS-PAGE)See COA
Accession #
P28066
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human PSMA5 Protein (rp192157) at 1.0 μg/mL can bind Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) with the EC50 of 148.2 ng/mL.
无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE),See COA
生化机理
PSMA5 (Proteasome subunit alpha type-5) belongs to the peptidase T1A family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMA5 has been shown to interact with PLK1. This peptidase has a very broad specificity for cleavage of peptide bonds. Recombinant human PSMA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
生物活性
Immobilized Recombinant Human PSMA5 Protein (rp192157) at 1.0 μg/mL can bind Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) with the EC50 of 148.2 ng/mL.
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human PSMA5 Protein (rp192157) - ELISA】
Immobilized Recombinant Human PSMA5 Protein (rp192157) at 1.0 μg/mL can bind Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) with the EC50 of 148.2 ng/mL.
Recombinant Human PSMA5 Protein (rp192157) - SDS-PAGE
2 μg/lane of Recombinant Human PSMA5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 27.0 kDa under reducing conditions and 27.2 kDa under non-reducing conditions.