级别和纯度: 无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥90%(SDS-PAGE)expressed in E. coli; 见COA
Ribosomal Protein L12 | L12 | 60S ribosomal protein L12 | Large ribosomal subunit protein uL11 | RPL12
规格或纯度
无载体,His标签,≥90%(SDS-PAGE),expressed in E. coli; 见COA
生化机理
Ribosomes, the organelles which catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are comprised of four RNA species and approximately 80 structurally distinct proteins. Ribosomal Protein L12 (RPL12) protein is a member of the L11P family of ribosomal proteins. RPL12 is a component of the 60S subunit, which is located in the cytoplasm. RPL12 binds directly to the 26S rRNA.
Post-translational: Ubiquitinated at Lys-48 and Lys-83 by RNF14 and RNF25 in response to ribosome collisions (ribosome stalling).
Recombinant Human RPL12 Protein (rp192202) - SDS-PAGE
3 μg/lane of Recombinant Human RPL12 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 18.6 kDa under reducing conditions and 17.7 kDa under non-reducing conditions.