This product is a freeze-dried powder of recombinant alkali resistant protein A expressed by Escherichia coli and purified by chromatography and freeze-drying. Aladdin recombinant protein A is a modification of the protein structure based on natural Staphylococcus aureus protein A. It consists of four Fc binding domain fragments linked together, with a molecular weight of 27KD. Compared with natural protein A, it removes the binding site with the immunoglobulin Fab region, enhances its binding ability with the Fc region, and improves the stability and alkali resistance of the protein. Recombinant protein A can be bonded to agarose gel to prepare an affinity filler for antibody purification. It can separate and purify antibodies of different subtypes of mammals or genetic engineering recombinant proteins containing Fc fragments of antibodies from ascites, serum, cell culture supernatant or cell extracts. The C-terminal of Aladdin recombinant alkali resistant protein A contains a cysteine, which can enhance its directional covalent bonding with gel. 2、Purpose Biochemical research: used for immune analysis and purification of IgG. Preparation of affinity fillers for antibody purification: Multiple mammalian subtypes of antibodies or genetically engineered recombinant proteins containing antibody Fc fragments can be isolated and purified from ascites, serum, cell culture supernatant, or cell extracts. 3、Recommended usage This product is a recombinant expression of alkali resistant protein A in Escherichia coli. Its C-terminus is designed with a cysteine residue, which can be coupled through the thiol non epoxy group on it to prepare affinity chromatography fillers. The activation of epoxy groups and protein A coupling methods need to be determined through experiments.
规格
产品名称
重组耐碱蛋白A
别名
重组蛋白A | 重组蛋白A | 重组葡萄球菌蛋白A
英文别名
Protein A | Staphylococcal Protein A | Staphylococcal Protein A Recombinant | Immunoglobulin G-binding protein A | gG-binding protein A | Immunoglobulin G binding protein A | SPA | rat-PA
规格或纯度
无动物源,无载体,PBS Only,≥90%(HPLC),Dimer≤2.0%(SEC-HPLC)
生化机理
Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins. In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
表达系统
E. coli
种属
金黄色葡萄球菌(Staphylococcus aureus)
蛋白标签
No tag
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
27 kDa
SDS-PAGE
24.3-29.7 kDa, under reducing conditions
CAS编号和信息
91932-65-9
分子类型
蛋白质
产品线
biological_products
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
50mM PBS, pH7.4
浓度
Dimer≤2.0%(SEC-HPLC)
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
ZJ25F0318840
危险品化学品经营许可证（带存储）