10-180kDa 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
1. 该产品包含了从10kDa到180kDa共10种高度纯化并预染的重组蛋白质。
2. 适合作为SDS-PAGE和Western的蛋白质分子量标准。
3. 本产品已经配制在1×SDS-PAGE上样缓冲液中，直接使用，不要煮沸、稀释和加入还原剂处理。
4. 根据上样孔的大小，本产品通常每次上样5-10微升（5×1.5mm胶孔5ul足够），即可在电泳时、电泳后和转膜后观察到非常清楚的蛋白条带。
预染蛋白质分子量标准经非预染蛋白质分子量标准标定，具有如下突出优势：
1. 蓝色和绿色条带在Tris-Glycine，Bis-Tris，Tris-Acetate缓冲体系SDS-PAGE胶其表观分子量基本没有变化。
2. 橙(红)色条带与进口产品变化一致。
使用方法：
1. 室温下解冻后完全溶解并轻轻充分混匀，不要煮沸。
2. 取本产品5µl与实验样品同时进行聚丙烯酰胺凝胶电泳；建议有条件的实验室在初次使用本产品时可以根据自身的实验条件和实验习惯通过预实验确定合适的上样量，这样可以节约成本，同时获得效果更佳的实验图片。
3. 未使用的产品保存于储存条件，在4°C可放置2个月。
注意事项：
1. 注意电泳时间，低分子量蛋白可能会泳动于染料前缘。
2. 大分子量蛋白Westernblot时需延长转膜时间或加高转膜电压。另外建议转膜液不添加SDS，若实验必须使用，建议SDS浓度不要超过0.02-0.04%。
3. 预染蛋白质在不同的缓冲体系下有不同的表观分子量，在该缓冲体系中事先用非预染蛋白质标定，可以大致确定蛋白质分子量。
Prestained Color Protein MW Marker (10-180kDa):
1. This product contains 10 highly purified and pre stained recombinant proteins ranging from 10kDa to 180kDa.
2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting.
3. This product has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents.
4. According to the size of the sample well, this product usually requires 5-10 microliters of sample to be loaded each time (5μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer.
The pre stained protein molecular weight standard, calibrated with non pre stained protein molecular weight standards, has the following outstanding advantages:
1. The blue and green bands showed little change in apparent molecular weight on SDS-PAGE gel in Tris Glycine, Bis Tris, and Tris Acetate buffer systems.
2. The orange (red) color stripes are consistent with the changes in imported products.
Usage method:
1. After thawing at room temperature, completely dissolve and gently mix well, do not boil.
2. Take 5µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images.
3. Unused product should be stored under storage conditions and can be left at 4°C for 2 months.
Precautions:
1. Pay attention to the electrophoresis time, as low molecular weight proteins may migrate to the front edge of the dye.
2. During Western blotting of high molecular weight proteins, it is necessary to extend the transfer time or increase the transfer voltage. Additionally, it is recommended not to add SDS to the transfer solution. If it is necessary for the experiment, it is suggested that the SDS concentration should not exceed 0.02-0.04%.
3. Pre stained proteins have different apparent molecular weights in different buffer systems. By pre calibrating with non pre stained proteins in this buffer system, the protein molecular weight can be roughly determined.
Prestained Color Protein MW Marker (10-180kDa) (rp192282) - SDS-PAGE band profile in Tris-Glycine
lmages are from a Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane. Prestained Color Protein MW Marker (10-180kDa) (rp192282) are mixture of 10 recombinant proteins, 8 blue-stained bands, a green-stained band (10kDa) and an orange-stained band (72kDa).
Prestained Color Protein MW Marker (10-180kDa) (rp192282) - SDS-PAGE band profile in Bis-Tris
lmages are from a Bis-Tris gel (SDS-PAGE) and subsequent transfer to membrane. Prestained Color Protein MW Marker (10-180kDa) (rp192282) are mixture of 10 recombinant proteins, 8 blue-stained bands, a green-stained band (10kDa) and an orange-stained band (65kDa).
(rp192282) - Migration patterns in different electrophoretic conditions
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein using the Prestained Color Protein MW Marker (10-180kDa) (rp192282) in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0636663 |
分析证书 | rp192282 |
| ZJ25F0724420 |
分析证书 | rp192282 |
| ZJ25F0623407 |
分析证书 | rp192282 |
| ZJ25F0622159 |
分析证书 | rp192282 |
| ZJ25F0521480 |
分析证书 | rp192282 |
| ZJ25F0420898 |
分析证书 | rp192282 |
| ZJ24F1114032 |
分析证书 | rp192282 |
| ZJ24F1013225 |
分析证书 | rp192282 |
| 1. Yiling Zhou, Yaru Liao, Jing Bai, Xinlei Xia, Zhihua Wu, Xin Li, Ping Tong, Hongbing Chen, Anshu Yang. (2024) Effects of active soybean isoflavones on the structure and potential allergenicity of glycinin. LWT-FOOD SCIENCE AND TECHNOLOGY, [10.1016/j.lwt.2024.116050] |
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