Bcl-X, also named as BCL2L1 or BCL2L, belongs to the Bcl-2 family and it is encoded by the BCL2L1 gene in human. Alternative splicing of Bcl-X results in at least two isoforms，isoform Bcl-X(L) (also named as Bcl-xL) and isoform Bcl-x(S) (also named as Bcl-xS). Bcl-xL is found in tissues containing long-lived postmitotic cells, such as adult brain, while Bcl-xS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. Bcl-X forms homodimer or heterodimer with other Bcl-2 proteins, like BAK, BAX or Bcl-2, to act as anti- or pro- apoptotic regulators. Bcl-xL appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane, and it also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. In contrast, Bcl-xS is a pro-apoptotic protein that promotes apoptosis.
Post-translational: Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis.Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome.
生物活性
Immobilized Recombinant Human BID Protein (rp183602) at 2.0 μg/mL can bind Recombinant Human Bcl-XL Protein (rp209934) with the EC50 of 261.4 ng/mL.
27.6 kDa, under reducing conditions; 27.6 & 53.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
ecombinant Human Bcl-XL Protein (rp209934) - Binding Activity Immobilized Recombinant Human BID Protein (rp183602) at 2.0 μg/mL can bind Recombinant Human Bcl-XL Protein (rp209934) with the EC
₅₀
of 261.4 ng/mL.
Recombinant Human Bcl-XL Protein (rp209934) - SDS-PAGE 3 μg/lane of Recombinant Human Bcl-XL Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 27.6 kDa under reducing conditions and 27.6 & 53.2 kDa under non-reducing conditions.
ZJ24F1215508
危险品化学品经营许可证（带存储）