Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >5 A650/min/mg, as measured under the described conditions. Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >5 A650/min/mg, as measured under the described conditions.
Thioredoxins (Trxs) are a group of small ubiquitous proteins in all living cells that are key regulators of cellular redox balance. The mammalian Trx family has three members: Trx-1, which is a secreted and cellular protein, the mitochondria-specific Trx-2, and the Trx-like cytosolic protein p32TrxL. The active site of mammalian Trxs contains two cysteines in the conserved sequence -Y-C-G-P-C-K-. In Trx-1, the conserved cysteine residues are in positions 32 and 35, respectively. Trxs exist either in a reduced or in an oxidized state when the two cysteines at the active site form an intramolecular disulfide bridge. NADPH and the flavoprotein thioredoxin reductase can convert the oxidized Trx into the reduced Trx. Trx-1 is the only extracellular occurring thioredoxin, and is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Plasma concentrations of Trx-1 are up to 6 nM. In cells, Trx-1 is localized predominantly in the cytoplasm. Small amounts have been detected in the nucleus and in association with the outside surface of the cells. Expression of Trx-1 is increased under various stress conditions such as hypoxia, elevated hydrogen peroxide concentrations, photochemical oxidative stress, and viral and bacterial infections. Biological functions of Trx-1 include growth factor activity, antioxidant properties, a cofactor that provides reducing equivalents, and transcriptional regulation. The synovial tissue of rheumatoid arthritis patients produces increased levels of Trx-1 under oxidative stress conditions, and a correlation exists between the plasma levels of Trx-1 and the severity of the disease, making Trx-1 a biomarker for this pathological condition.
生物活性
Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >5 A650/min/mg, as measured under the described conditions. Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >5 A650/min/mg, as measured under the described conditions.
39.6 & 90.8 kDa, under reducing conditions; 38.9 & 41.3 & 92.1 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM Tris, 100mM NaCl, pH 8.0
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant GST-WELQ-HA-TrxA Protein (rp213144) - SDS-PAGE 3 μg/lane of Recombinant GST-WELQ-HA-TrxA Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 39.6 & 90.8 kDa under reducing conditions and 38.9 & 41.3 & 92.1 kDa under non-reducing conditions.
Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >5 A650/min/mg, as measured under the described conditions.
Materials:
Buffer : 50 mM MES, 250 mM NaCl, 2 Mm EDTA, pH 6.5
Substrate: Recombinant Human Insulin Protein (rp218456)
Dithiothreitol (DTT) ( B104860)
Procedures：
1. Dilute Recombinant GST-WELQ-HA-TrxA Protein (rp213144) to 6.9 µM in Assay Buffer.
2. Dilute Recombinant Human Insulin Protein (rp218456) to 1040 µM µM in Assay Buffer.
3. Dilute DTT to 55 mM in Assay Buffer.
4. Load into a clear plate 50 µL of Assay Buffer, 25 µL of Recombinant GST-WELQ-HA-TrxA Protein (rp213144) , and 25 µL of Recombinant Human Insulin Protein (rp218456). For a Substrate Blank, load 75 µL of Assay Buffer and 25 µL of Recombinant Human Insulin Protein (rp218456). Start the reaction by adding 10 µL of 55 mM DTT to all wells.
5. Read at Abs650 in kinetic mode for 30 minutes. Use the linear portion of the reaction to determine specific activity.
Calculate specific activity: Specific Activity (Abs/min/mg) =Adjusted Vmax* (Abs/min)**/
amount of enzyme (mg)
Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >5 A650/min/mg, as measured under the described conditions.
Materials:
Buffer : 50 mM MES, 250 mM NaCl, 2 Mm EDTA, pH 6.5
Substrate:Recombinant Human Insulin Protein (rp218456)
Dithiothreitol (DTT) ( B104860)
Procedures：
1. Dilute Recombinant GST-WELQ-HA-TrxA Protein (rp213144) in Assay Buffer.
2. Dilute Recombinant Human Insulin Protein (rp218456) to 1040 µM µM in Assay Buffer.
3. Dilute DTT to 55 mM in Assay Buffer.
4. Load into a clear plate 50 µL of Assay Buffer, 25 µL of Recombinant GST-WELQ-HA-TrxA Protein (rp213144) , and 25 µL ofRecombinant Human Insulin Protein (rp218456). For a Substrate Blank, load 75 µL of Assay Buffer and 25 µL ofRecombinant Human Insulin Protein (rp218456). Start the reaction by adding 10 µL of 55 mM DTT to all wells.
5. Read at Abs650 in kinetic mode for 30 minutes. Use the linear portion of the reaction to determine specific activity.
Calculate specific activity: Specific Activity (Abs/min/mg) =Adjusted Vmax* (Abs/min)**/
amount of enzyme (mg)
ZJ24F1216142
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