Immobilized Recombinant Human GRP78/HSPA5 Protein (rp214409) at 1.0 μg/mL can bind Recombinant GRP78 BiP Antibody (Ab106527) with the EC50 of 13.1 ng/mL.
78 kDa glucose regulated protein | 78 kDa glucose-regulated protein | AL022860 | AU019543 | BIP | D2Wsu141e | D2Wsu17e | Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 | Endoplasmic reticulum lumenal Ca2+ binding protein grp78 | Epididymis sec
规格或纯度
无动物源,无载体,生物活性,His标签,≥95%(SDS-PAGE),见COA
生化机理
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Post-translational: AMPylated by FICD (PubMed:25601083). In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins (By similarity). In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity (By similarity).
生物活性
Immobilized Recombinant Human GRP78/HSPA5 Protein (rp214409) at 1.0 μg/mL can bind Recombinant GRP78 BiP Antibody (Ab106527) with the EC50 of 13.1 ng/mL.
Recombinant Human GRP78/HSPA5 Protein (rp214409) - ELISA Immobilized Recombinant Human GRP78/HSPA5 Protein (rp214409) at 1.0 μg/mL can bind Recombinant GRP78 BiP Antibody (Ab106527) with the EC₅₀ of 13.1 ng/mL.
Recombinant Human GRP78/HSPA5 (rp214409) - SDS-PAGE 3 μg/lane of Recombinant Human GRP78/HSPA5 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 66.0 kDa under reducing conditions and 66.4 kDa under non-reducing conditions.