Recombinant Human Myoglobin Protein

货号: rp214420
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 ≥95%(SDS-PAGE) 见COA
Accession #
P02144
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human Myoglobin Protein (rp214420) at 1.0 μg/mL can bind Recombinant Myoglobin Antibody (Ab007432) with the EC50 of 4.7 ng/mL.
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规格
库存
价格
数量
10μg
rp214420-10μg
期货 Stock Image
¥369.90
50μg
rp214420-50μg
期货 Stock Image
¥1,299.90
100μg
rp214420-100μg
期货 Stock Image
¥1,999.90
1mg
rp214420-1mg
期货 Stock Image
¥12,999.90
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为什么选择此级别

ActiBioPure™,His标签,无载体,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Human Myoglobin Protein
别名
重组人Myoglobin蛋白 | 重组人肌红蛋白
英文别名
MB | MGC13548 | MYG_HUMAN | Myoglobin
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE),见COA
生化机理
Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed:30918256, PubMed:34679218). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide (PubMed:32891753). Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (PubMed:34679218).
生物活性
Immobilized Recombinant Human Myoglobin Protein (rp214420) at 1.0 μg/mL can bind Recombinant Myoglobin Antibody (Ab007432) with the EC50 of 4.7 ng/mL.
表达系统
E. coli
种属
人(Human)
氨基酸
2-154 aa
序列
MHHHHHHGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG
蛋白标签
N-His
无载体
Yes
无动物源
No
来源
重组表达
预测分子量
18.0 kDa
SDS-PAGE
17.4 kDa, under reducing conditions; 16.8 & 17.5 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
Lyophilized from 20mM Tris, 300mM NaCl, pH 8.0
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃ 存储6个月;收货后建议分装,避免反复冻融。
图片

Recombinant Human Myoglobin Protein (rp214420) - ELISA
Immobilized Recombinant Human Myoglobin Protein (rp214420) at 1.0 μg/mL can bind Recombinant Myoglobin Antibody (Ab007432) with the EC50 of 4.7 ng/mL.

Recombinant Human Myoglobin Protein (rp214420) - SDS-PAGE
3 μg/lane of Recombinant Human Myoglobin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 17.4 kDa under reducing conditions and 16.8 & 17.5 kDa under non-reducing conditions.
Myoglobin is a protein consisting of a single alpha-helical structure containing a Heme group. It reversibly binds oxygen through pentacorated heme iron and releases oxygen in a timely and efficient manner as needed during periods of increased demand. The main reason for the existence of two conformations in the non-reducing state is related to the different spin states and coordination modes of iron (Fe) in the heme cogroup. (PubMed:30918256, PubMed:34679218)

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器