无动物源, 无载体, His标签, ≥95%(SDS-PAGE), expressed in HEK293; 见COA
生化机理
Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. It is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Mouse IL-12p40 is a 40 kDa glycoprotein that shows considerable structural similarity to the extracellular domain of hematopoietin receptors. It is synthesized as a 335 amino acid (aa) precursor with a 22 aa signal sequence and a 313 aa mature region that contains a 92 aa fibronectin type III domain and an 84 aa Ig C2-like region and has a high degree of structural homology to type I cytokine receptors. There are two intrachain disulfide bonds and four potential N-linked glycosylation sites. Once made, it can exist in multiple forms including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). Mature mouse IL-12p40 shows 93% and 67% aa sequence identity to rat and human IL-12p40 respectively. The secreted form of the p40 subunit inhibits IL-23 functions and abrogates IL-23-mediated anti-tumor effects. Characterization of the IL-12p40 proteins for binding and bioactivity showed that both the p40 monomer and dimer inhibited IL-12 binding to IL-12R.
41.9-53.3 kDa, under reducing conditions; 38.7-49.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Mouse IL-12B Protein (rp214431) - SDS-PAGE
3 μg/lane of Recombinant Mouse IL-12 p40/IL-12B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 41.9-53.3 kDa under reducing conditions and 38.7-49.4 kDa under non-reducing conditions.
ZJ26F0535874
危险品化学品经营许可证（带存储）