级别和纯度: 无动物源?无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥90%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
P27797
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human Calreticulin Protein (rp214470) at 1.0 μg/mL can bind Recombinant Calreticulin Antibody (Ab092591) with the EC50 of 7.01 ng/mL.
无动物源, 无载体, 生物活性, ActiBioPure™, His标签, ≥90%(SDS-PAGE), expressed in HEK293; 见COA
生化机理
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246).
Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926).
Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
生物活性
Immobilized Recombinant Human Calreticulin Protein (rp214470) at 1.0 μg/mL can bind Recombinant Calreticulin Antibody (Ab092591) with the EC50 of 7.01 ng/mL.
54.0 & 59.6 kDa, under reducing conditions; 54.3 & 59.6 & 114.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human Calreticulin Protein (rp214470) - ELISA
Immobilized Recombinant Human Calreticulin Protein (rp214470) at 1.0 μg/mL can bind Recombinant Calreticulin Antibody (Ab092591) with the EC50 of 7.01 ng/mL.
Recombinant Human Calreticulin Protein (rp214470) - SDS-PAGE
3 μg/lane of Recombinant Human Calreticulin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 54.0 & 59.6 kDa under reducing conditions and 54.3 & 59.6 & 114.7 kDa under non-reducing conditions.
Under specific non-physiological conditions, including high concentrations or particular buffer environments, Calreticulin/CALR monomers have the potential to form non-covalent dimers or higher-order oligomers through non-covalent interactions (Uniprot ID: P27797).