计算溶液所需的质量、体积或浓度。
BioReagent,EnzymoPure™,for DNA and RNA applications,PCR试剂,分子生物学级,用于DNA合成,用于体外转录 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
储存缓冲液: 50mM Tris, 50mM KCl, 1mM DTT, 0.05mM EDTA, 50% Glycerol, 200 μg/ml HSA, pH 8.0
Exonuclease III 具有作用于双链 DNA 的 3’→5’ 外切酶活性,能降解平末端、3’ 末端凹陷及有切口的 DNA,从 3’ 端降解 DNA 分子。对于 3´ 突出末端,特别是突出 4 nt 或更长的DNA 分子完全不能被切割。此外,该酶的活性部位依赖螺旋结构,并根据序列的不同有差异(C>A=T>G)。
产品组分
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来源
E.coli
酶活定义
在 50 μl 反应体系中,37℃ 条件下,30 min催化产生 1 nmol 酸溶性总核苷酸所需要的酶量定义为 1 个单位(U)。
产品应用
(1)非定向巢式缺失;
(2)定点突变;
(3)链特异性探针的制备;
(4)制备用于双脱氧测序的单链底物。
产品使用步骤
(1)配置下列反应体系
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(2)37“C孵育 30min。
(3)75°C孵育 10 min 终止反应。
注意事项
(1)反应温度、体系中盐离子浓度以及酶与 DNA 的比例都会影响酶的活性。
(2)本产品仅作科学研究使用,不得用于其它用途。
Storage buffer: 50mM Tris, 50mM KCl, 1mM DTT, 0.05mM EDTA, 50% Glycerol, 200 μg/ml HSA, pH 8.0
Exonuclease III has 3 '→5' exonuclease activity on double-stranded DNA, which can degrade flat end, 3 'end dented and incised DNA, and degrade DNA molecules from the 3' end. For 3 'protruding ends, especially protruding DNA molecules 4 nt or longer cannot be cut at all. In addition, the active site of the enzyme depends on the helical structure and varies according to the sequence (C>A=T>G).
Product composition:
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Source
E.coli
Enzyme Activity Definition
The amount of enzyme required to catalyze the production of 1 nmol acid-soluble total nucleotide in a 50 μl reaction system at 37℃ for 30 min is defined as 1 unit (U).
Applications
(1) Non-directional nested deletion;
(2) Site-directed mutagenesis;
(3) Preparation of chain specific probes;
(4) Preparation of single-stranded substrates for dideoxy sequencing.
Protocol
(1) Configure the reaction system, as shown in the following table
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(2) Incubation at 37 "C for 30min.
(3) Incubation at 75°C for 10 min terminated the reaction.
Cautions
(1) The reaction temperature, the concentration of salt ions in the system and the ratio of enzyme to DNA will affect the activity of the enzyme.
(2) This product is for scientific research only and shall not be used for other purposes.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp216608 | |
| 分析证书 | rp216608 | |
| 分析证书 | rp216608 |